Arginase activity of ovarian structures in cows of Brown Swiss and its cross-breeds/Aktivnost arginaze u ovarijskim strukturama krava svicarske smede pasmine i njezinih krizanaca

• Published in: Veterinarski arhiv p. 261
• Main Authors: Aksu, Emrah H, Kandemir, Fatih M, Kilic, Kubra, Akman, Orhan, Omur, Ali D, Ucar, Omer
• Format: Journal Article
• Language: English
• Published: Sveuciliste U Zagrebu 01.05.2015
• Subjects: Switzerland
• ISSN: 0372-5480; 1331-8055

Arginase is the last enzyme of the urea cycle. It catalyses the hydrolysis of L-arginine to L-ornitine and urea. The aim of this study was to investigate the presence of arginase activity in ovarian structures such as: Graaf follicles, GF (Medium and Large size, M- and L-size), Corpus Haemorrhagicum (CH), and various types of Corpus Luteum (CL) such as: cyclic CL (CCL), 2-4 month pregnancy CL (2-4 MCL) and 4-7 month pregnancy CL (4-7 MCL). Ovarian tissues of 62 cows (7-10 years old and Brown Swiss or its cross-breeds), collected from a local slaughterhouse, were used as material. The materials were divided into 6 experimental groups, as follows: MGF group (n = 7), LGF group (n = 21), CH group (n = 7), CCL group (n = 6), 2-4 MCL group (n = 9) and 4-7 MCL group (n = 12). Arginase activities were measured as 0.056 ± 0.017, 0.100 ± 0.016, 2.517 ± 0.521, 0.827 [+ or -] 0.190, 0.674 ± 0.106 and 0.833 ± 0.093 U/mg protein in all groups, respectively. Arginase activity in the CH group was significantly higher than that in the CCL, 2-4 MCL and 4-7 MCL groups (P<0.001). The lowest enzyme activity was in the MGF and LGF groups. Hence, it was concluded that the arginase enzyme might play a crucial role in cell division, proliferation and differentiation in the ovarian tissues (especially the CH) of mature cows. Key words: arginase activity, Graaf follicle, corpus heamorrhagicum, corpus luteum, cattle Arginaza je posljednji enzim u ciklusu ureje koji katalizira hidrolizu L-arginina u L-ornitin i ureju. Cilj istrazivanja bio je utvrditi prisutnost aktivnosti arginaze u ovarijskim strukturama kao sto su Graafovi folikuli, GF (srednje veliki--M i veliki--L), corpus haemorrhagicum (CH) i razliciti tipovi corpus luteum (CL) kao sto su ciklicni (CCL), 2-4 mjeseca graviditetni (2-4 MCL) i 4-7 mjeseci graviditetni (4-7 MCL). Tkiva ovarija od 62 krave (svicarske smede pasmine i krizanaca u dobi od 7 do 10 godina) prikupljena su u lokalnoj klaonici. Materijali su bili podijeljeni u 6 pokusnih skupina kako slijedi: MGF skupina (n = 7), LGF skupina (n = 21), CH skupina (n = 7), CCL skupina (n = 6), 2-4 MCL skupina (n = 9) i 4-7 MCL skupina (n = 12). Slijedom navedenih skupina, aktivnost arginaze bila je 0,056 ± 0,017, 0,100 ± 0,016, 2,517 ± 0,521, 0,827 [+ or -] 0,190, 0,674 ± 0,106 i 0,833 ± 0,093 U/mg. Aktivnost arginaze u CH skupini bila je signifikantno visa u odnosu na skupine CCL, 2-4 MCL i 4-7 MCL (P < 0,001). Najniza aktivnost enzima bila je u skupinama MGF i LGF. Zakljuceno je o mogucoj kljucnoj ulozi aktivnosti enzima arginaze u diobi stanica, proliferaciji i diferencijaciji ovarijskih tkiva (osobito CH) kod odraslih krava. Kljucne rijeci: aktivnost arginaze, Graafov folikul, corpus heamorrhagicum, corpus luteum, govedo