A IPrunus avium/I L. Infusion Inhibits Sugar Uptake and Counteracts Oxidative Stress-Induced Stimulation of Glucose Uptake by Intestinal Epithelial Cells

Sweet cherry (Prunus avium L.) is among the most valued fruits due to its organoleptic properties and nutritional worth. Cherry stems are rich in bioactive compounds, known for their anti-inflammatory and antioxidant properties. Innumerable studies have indicated that some bioactive compounds can mo...

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Published inAntioxidants Vol. 13; no. 1
Main Authors Barreto-Peixoto, Juliana A, Silva, Cláudia, Costa, Anabela S. G, Álvarez-Rivera, Gerardo, Cifuentes, Alejandro, Ibáñez, Elena, Oliveira, M. Beatriz P. P, Alves, Rita C, Martel, Fátima, Andrade, Nelson
Format Journal Article
LanguageEnglish
Published MDPI AG 01.12.2023
Subjects
Antioxidants
Dextrose
Ethylenediaminetetraacetic acid
Fructose
Gene expression
Glucose
Glucose metabolism
Instrument industry
International economic relations
Metabolites
Oxidative stress
Resveratrol
Type 2 diabetes
United States
Portugal
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Summary:Sweet cherry (Prunus avium L.) is among the most valued fruits due to its organoleptic properties and nutritional worth. Cherry stems are rich in bioactive compounds, known for their anti-inflammatory and antioxidant properties. Innumerable studies have indicated that some bioactive compounds can modulate sugar absorption in the small intestine. In this study, the phenolic profile of a cherry stem infusion was investigated, as well as its capacity to modulate intestinal glucose and fructose transport in Caco-2 cells. Long-term (24 h) exposure to cherry stem infusion (25%, v/v) significantly reduced glucose ([sup.3]H-DG) and fructose ([sup.14]C-FRU) apical uptake, reduced the apical-to-basolateral P[sub.app] to [sup.3]H-DG, and decreased mRNA expression levels of the sugar transporters SGLT1, GLUT2 and GLUT5. Oxidative stress (induced by tert-butyl hydroperoxide) caused an increase in [sup.3]H-DG uptake, which was abolished by the cherry stem infusion. These findings suggest that cherry stem infusion can reduce the intestinal absorption of both glucose and fructose by decreasing the gene expression of their membrane transporters. Moreover, this infusion also appears to be able to counteract the stimulatory effect of oxidative stress upon glucose intestinal uptake. Therefore, it can be a potentially useful compound for controlling hyperglycemia, especially in the presence of increased intestinal oxidative stress levels.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox13010059