Antioxidative metabolism in sugarcane varieties subjected to water and saline stress/Metabolismo antioxidativo em variedades de cana-de-acucar sob estresse hidrico e salino

This study aimed to identify the antioxidant responses of sugarcane (Saccharum spp.) varieties subjected to water and saline stress. Sugarcane seedlings of six different varieties obtained through micropropagation were subjected to either water or saline stress, or a combination of water + saline st...

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Published inRevista brasileira de engenharia agrícola e ambiental Vol. 24; no. 11; p. 776
Main Authors Morais, Marciana B. de, Azevedo, Ricardo A, Camara, Terezinha R, Ulisses, Claudia, Albuquerque, Cynthia C. de, Willadino, Lilia
Format Journal Article
LanguageEnglish
Published ATECEL--Associacao Tecnico Cientifica Ernesto Luiz de Oliveira Junior 01.11.2020
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Summary:This study aimed to identify the antioxidant responses of sugarcane (Saccharum spp.) varieties subjected to water and saline stress. Sugarcane seedlings of six different varieties obtained through micropropagation were subjected to either water or saline stress, or a combination of water + saline stress. The study was carried out in May 2012, in a greenhouse at the Universidade Federal Rural de Pernambuco (UFRPE). The experimental design was randomized, with treatments arranged in a 6 x 4 factorial scheme (six varieties and four treatments), and four replicates. Lipid peroxidation, hydrogen peroxide (H20 ) concentrations, and relative water content (RWC) were evaluated. Furthermore, we evaluated the plants' antioxidative defense systems by measuring the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT). The sugarcane varieties had higher lipid peroxidation and/or higher H2O2 concentrations when subjected to the combined water + saline stress. The antioxidant enzymes responded to the water and saline stress treatments differently depending on the sugarcane variety. However, under combined saline + water stress conditions, the enzymes may have become inactivated, which indicates that the response to the combined water + saline stress was different from the sum of the responses to only water stress or only saline stress. High concentrations of malondialdehyde (MDA) associated with low RWC maybe an effective indicator of multiple stress sensitivity in sugarcane varieties. The RB99395 and RB867515 sugarcane varieties responded more efficiently to environmental stress, and maintained their cell water content when subjected to either water or saline stress. Keywords: Sachorum, oxidative stress, defense system, ROS Objetivou-se com este trabalho identificar a resposta antioxidante de variedades de cana-de-acucar sob condicoes adversas. Mudas de seis genotipos, procedentes da micropropagacao, foram submetidas a avaliacao da acao isolada e combinada do estresse hidrico e salino. A pesquisa foi realizada em maio de 2012, em casa de vegetacao instalada na Universidade Federal Rural de Pernambuco (UFRPE). O delineamento experimental foi o inteiramente casualizado, com tratamentos dispostos em esquema fatorial 6x4 (seis variedades e quatro condicoes de cultivo) com quatro repeticoes. Avaliou-se a peroxidacao lipidica, o teor de peroxido de hidrogenio ([H.sub.2O.sub.2]), o teor relativo de agua (TRA) e acao do sistema de defesa antioxidativo mediante aatividade das enzimas superoxido dismutase (SOD), ascorbato peroxidase (APX) e catalase (CAT). As variedades de cana-de-acucar exibiram maior peroxidacao lipidica e/ou maior concentracao de peroxido de hidrogenio quando submetidas ao estresse combinado. As enzimas apresentaram respostas diferenciadas de acordo com a variedade de cana-de-acucar e a intensidade de estresse nos tratamentos isolados, no entanto, podem ter sido inativadas perante o estresse combinado, o que indica que a resposta do estresse combinado e diferente da soma das respostas dos fatores abioticos aplicados isoladamente. Os elevados teores de malondialdeido (MDA) associados ao baixo TRA podem ser indicadores efetivos de sensibilidade a estresse multiplo em variedades de cana-de-acucar. As variedades RB99395 e RB867515 apresentaram-se mais eficientes quanto a resposta ao estresse ambiental e a manutencao de conteudo hidrico celular nos estresses isolados. Palavras-chave: Sochorum, estresse oxidativo, sistema de defesa, ROS
ISSN:1415-4366
1807-1929
DOI:10.1590/1807-1929/agriambi.v24nllp776-782