Surface-engineered Saccharomyces cerevisiae displaying [alpha]-acetolactate decarboxylase from Acetobacter aceti ssp xylinum

• Published in: Biotechnology letters Vol. 38; no. 12; p. 2145
• Main Authors: Cejnar, Rudolf, Hlozková, Katerina, Kotrba, Pavel, Dostálek, Pavel
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01.12.2016
• Subjects: Bacteria; Beer; Biotechnology; Enzymes; Fermentation; Yeast
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-016-2205-1

Objectives To convert [alpha]-acetolactate into acetoin by an [alpha]-acetolactate decarboxylase (ALDC) to prevent its conversion into diacetyl that gives beer an unfavourable buttery flavour. Results We constructed a whole Saccharomyces cerevisiae cell catalyst with a truncated active ALDC from Acetobacter aceti ssp xylinum attached to the cell wall using the C-terminal anchoring domain of [alpha]-agglutinin. ALDC variants in which 43 and 69 N-terminal residues were absent performed equally well and had significantly decreased amounts of diacetyl during fermentation. With these cells, the highest concentrations of diacetyl observed during fermentation were 30 % less than those in wort fermented with control yeasts displaying only the anchoring domain and, unlike the control, virtually no diacetyl was present in wort after 7 days of fermentation. Conclusions Since modification of yeasts with ALDC variants did not affect their fermentation performance, the display of [alpha]-acetolactate decarboxylase activity is an effective approach to decrease the formation of diacetyl during beer fermentation.