Method Based on the [beta]-Lactamase PenPC Fluorescent Labeled for [beta]-Lactam Antibiotic Quantification in Human Plasma
Recently, Wong et al. have successfully developed a fluorescent biosensor based on the PenPC β-lactamase which changes its intrinsic fluorescence in presence of β-lactam antibiotics (BLAs). Here, we studied systematically this correlation among the fluorescence change of the biosensor and the concen...
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| Published in | BioMed research international Vol. 2016 |
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| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
New York
John Wiley & Sons, Inc
01.01.2016
Hindawi Limited |
| Subjects | |
| Online Access | Get full text |
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| Abstract | Recently, Wong et al. have successfully developed a fluorescent biosensor based on the PenPC β-lactamase which changes its intrinsic fluorescence in presence of β-lactam antibiotics (BLAs). Here, we studied systematically this correlation among the fluorescence change of the biosensor and the concentration of different BLAs aimed at developing a novel method for estimating the concentration of a wide range of BLAs. This method showed high precision and specificity and very low interference from clinically relevant samples. We were able to monitor the pharmacokinetics of meropenem in healthy volunteers as well as in an ill animal model too, indicating that the implemented method could be suitable for clinical practice. |
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| AbstractList | Recently, Wong et al. have successfully developed a fluorescent biosensor based on the PenPC β-lactamase which changes its intrinsic fluorescence in presence of β-lactam antibiotics (BLAs). Here, we studied systematically this correlation among the fluorescence change of the biosensor and the concentration of different BLAs aimed at developing a novel method for estimating the concentration of a wide range of BLAs. This method showed high precision and specificity and very low interference from clinically relevant samples. We were able to monitor the pharmacokinetics of meropenem in healthy volunteers as well as in an ill animal model too, indicating that the implemented method could be suitable for clinical practice. |
| Audience | Academic |
| Author | Alegria, Leyla Silva, Camila Andresen-Vasquez, Max Wong, Wai-Ting Wong, Kwok-Yin Chan, Pak-Ho Soto, Dagoberto Tapia, Pablo Leung, Yun-Chung Downey, Patricio Andresen, Max |
| Author_xml | – sequence: 1 fullname: Andresen, Max – sequence: 2 fullname: Wong, Kwok-Yin – sequence: 3 fullname: Leung, Yun-Chung – sequence: 4 fullname: Wong, Wai-Ting – sequence: 5 fullname: Chan, Pak-Ho – sequence: 6 fullname: Andresen-Vasquez, Max – sequence: 7 fullname: Alegria, Leyla – sequence: 8 fullname: Silva, Camila – sequence: 9 fullname: Tapia, Pablo – sequence: 10 fullname: Downey, Patricio – sequence: 11 fullname: Soto, Dagoberto |
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| Copyright | COPYRIGHT 2016 John Wiley & Sons, Inc. Copyright © 2016 Max Andresen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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| Snippet | Recently, Wong et al. have successfully developed a fluorescent biosensor based on the PenPC β-lactamase which changes its intrinsic fluorescence in presence... |
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| SubjectTerms | Analysis Antibiotics Beta lactam antibiotics Beta lactamases Biomedical research Clinical medicine Detectors Ethics Fluorescence Laboratory animals Methods |
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| Title | Method Based on the [beta]-Lactamase PenPC Fluorescent Labeled for [beta]-Lactam Antibiotic Quantification in Human Plasma |
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