Method Based on the [beta]-Lactamase PenPC Fluorescent Labeled for [beta]-Lactam Antibiotic Quantification in Human Plasma

Recently, Wong et al. have successfully developed a fluorescent biosensor based on the PenPC β-lactamase which changes its intrinsic fluorescence in presence of β-lactam antibiotics (BLAs). Here, we studied systematically this correlation among the fluorescence change of the biosensor and the concen...

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Bibliographic Details
Published inBioMed research international Vol. 2016
Main Authors Andresen, Max, Wong, Kwok-Yin, Leung, Yun-Chung, Wong, Wai-Ting, Chan, Pak-Ho, Andresen-Vasquez, Max, Alegria, Leyla, Silva, Camila, Tapia, Pablo, Downey, Patricio, Soto, Dagoberto
Format Journal Article
LanguageEnglish
Published New York John Wiley & Sons, Inc 01.01.2016
Hindawi Limited
Subjects
Analysis
Antibiotics
Beta lactam antibiotics
Beta lactamases
Biomedical research
Clinical medicine
Detectors
Ethics
Fluorescence
Laboratory animals
Methods
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Summary:Recently, Wong et al. have successfully developed a fluorescent biosensor based on the PenPC β-lactamase which changes its intrinsic fluorescence in presence of β-lactam antibiotics (BLAs). Here, we studied systematically this correlation among the fluorescence change of the biosensor and the concentration of different BLAs aimed at developing a novel method for estimating the concentration of a wide range of BLAs. This method showed high precision and specificity and very low interference from clinically relevant samples. We were able to monitor the pharmacokinetics of meropenem in healthy volunteers as well as in an ill animal model too, indicating that the implemented method could be suitable for clinical practice.
ISSN:2314-6133
2314-6141
DOI:10.1155/2016/4307987