Differential Adaptation of Human Gut Microbiota to Bariatric Surgery-Induced Weight Loss: Links With Metabolic and Low-Grade Inflammation Markers

• Published in: Diabetes (New York, N.Y.) Vol. 59; no. 12; pp. 3049 - 3057
• Main Authors: FURET, Jean-Pierre, KONG, Ling-Chun, RIZKALLA, Salwa, CLEMENT, Karine, TAP, Julien, POITOU, Christine, BASDEVANT, Arnaud, BOUILLOT, Jean-Luc, MARIAT, Denis, CORTHIER, Gérard, DORE, Joël, HENEGAR, Corneliu
• Format: Journal Article
• Language: English
• Published: Alexandria, VA American Diabetes Association 01.12.2010
• Subjects: Adaptation, Physiological; Analysis; Bacteroides; Bacteroides - genetics; Bacteroides - isolation & purification; Bariatric Surgery; Bifidobacterium; Bifidobacterium - genetics; Bifidobacterium - isolation & purification; Biological and medical sciences; Blood Glucose - metabolism; Body composition; Body fat; Calories; Care and treatment; Clostridium - genetics; Clostridium - isolation & purification; Diabetes; Diabetes. Impaired glucose tolerance; DNA Primers; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Escherichia coli; Escherichia coli - isolation & purification; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Faecalibacterium prausnitzii; Feces; Feces - microbiology; Female; Food; Gastrointestinal surgery; Glucose; Humans; Inflammation; Inflammation - etiology; Inflammation - microbiology; Inflammation - physiopathology; Insulin; Lactobacillus; Lactobacillus - isolation & purification; Leptin; Leuconostoc; Leuconostoc - isolation & purification; Life Sciences; Male; Medical sciences; Metabolism; Microbiota; Microbiota (Symbiotic organisms); Obesity; Obesity - microbiology; Obesity Studies; Patient outcomes; Pediococcus; Pediococcus - isolation & purification; Physiological aspects; Polymerase Chain Reaction; Prevotella; Research design; Starvation - microbiology; Surgery; Thinness - microbiology; Weight control; Weight loss; Weight Loss - physiology; France; Endocrinopathy; Human; Digestive system; Surgery; Diabetes mellitus; Gut; Weight loss; Microflora; Inflammation; Adaptation; LEPTIN; GASTRIC BYPASS-SURGERY; ENERGY; PEPTIDE-YY; DIET; MORBIDLY OBESE SUBJECTS; ENDOTOXEMIA; HUMAN COLONIC MICROBIOTA; INSULIN SENSITIVITY; ADIPOSE-TISSUE
• ISSN: 0012-1797; 1939-327X; 1939-327X
• DOI: 10.2337/db10-0253

Obesity alters gut microbiota ecology and associates with low-grade inflammation in humans. Roux-en-Y gastric bypass (RYGB) surgery is one of the most efficient procedures for the treatment of morbid obesity resulting in drastic weight loss and improvement of metabolic and inflammatory status. We analyzed the impact of RYGB on the modifications of gut microbiota and examined links with adaptations associated with this procedure. Gut microbiota was profiled from fecal samples by real-time quantitative PCR in 13 lean control subjects and in 30 obese individuals (with seven type 2 diabetics) explored before (M0), 3 months (M3), and 6 months (M6) after RYGB. Four major findings are highlighted: 1) Bacteroides/Prevotella group was lower in obese subjects than in control subjects at M0 and increased at M3. It was negatively correlated with corpulence, but the correlation depended highly on caloric intake; 2) Escherichia coli species increased at M3 and inversely correlated with fat mass and leptin levels independently of changes in food intake; 3) lactic acid bacteria including Lactobacillus/Leuconostoc/Pediococcus group and Bifidobacterium genus decreased at M3; and 4) Faecalibacterium prausnitzii species was lower in subjects with diabetes and associated negatively with inflammatory markers at M0 and throughout the follow-up after surgery independently of changes in food intake. These results suggest that components of the dominant gut microbiota rapidly adapt in a starvation-like situation induced by RYGB while the F. prausnitzii species is directly linked to the reduction in low-grade inflammation state in obesity and diabetes independently of calorie intake.