The IL-6 Signal Transducer, gp130: An Oncostatin M Receptor and Affinity Converter for the LIF Receptor

• Published in: Science (American Association for the Advancement of Science) Vol. 255; no. 5050; pp. 1434 - 1437
• Main Authors: Gearing, David P., Comeau, Michael R., Friend, Della J., Gimpel, Steven D., Thut, Catherine J., McGourty, Jackie, Brasher, Kelle K., King, Julie A., Gillis, Steven, Mosley, Bruce, Ziegler, Steven F., Cosman, David
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 13.03.1992; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Animals; Antigens, CD; Binding sites; Binding, Competitive; Biological and medical sciences; Blogs; CDNA libraries; Cell Line, Transformed; Cell receptors; Cell structures and functions; Cellular biology; Complementary DNA; COS cells; Cytokine Receptor gp130; Cytokines; Deoxyribonucleic acid; DNA; Embryonic cells; Fundamental and applied biological sciences. Psychology; genes; glycoprotein gp130; Growth Inhibitors - metabolism; Individualized Instruction; Interleukin-6; Interleukin-6 - metabolism; Leukemia; Leukemia Inhibitory Factor; Ligands; Lymphokines - metabolism; Medical research; Membrane Glycoproteins - metabolism; Mice; Miscellaneous; Molecular and cellular biology; Oncostatin M; Peptides - metabolism; Radioligand Assay; Receptors; Receptors, Cytokine; Receptors, Immunologic - metabolism; Receptors, OSM-LIF; Recombinant Proteins - metabolism; signal transduction; Transfection; Cytokine; Monkey; Interleukin 6; Signal transduction; Competitive fixation; Vertebrata; Mammalia; Cell line; Primates; Affinity; Kinetic parameter; Fibroblast; Biological receptor
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1542794

Leukemia inhibitory factor (LIF) and interleukin-6 (IL-6) are multifunctional cytokines with many similar activities. LIF is structurally and functionally related to another cytokine, Oncostatin M (OSM), that binds to the high-affinity LIF receptor but not to the low-affinity LIF receptor. A complementary DNA was isolated that encodes the high-affinity converting subunit of the LIF receptor. The converter conferred high-affinity binding of both LIF and OSM when expressed with the low-affinity LIF receptor and is identical to the signal transducing subunit of the IL-6 receptor, gp130. The gp130 subunit alone confers low-affinity binding of OSM when expressed in COS-7 cells. This receptor system resembles the high-affinity receptors for granulocyte-macrophage colony-stimulating factor, IL-3, and IL-5, which share a common subunit.