Talin Binding to Integrin β Tails: A Final Common Step in Integrin Activation

• Published in: Science (American Association for the Advancement of Science) Vol. 302; no. 5642; pp. 103 - 106
• Main Authors: Tadokoro, Seiji, Shattil, Sanford J., Eto, Koji, Tai, Vera, Liddington, Robert C., de Pereda, José M., Ginsberg, Mark H., Calderwood, David A.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Association for the Advancement of Science 03.10.2003
• Subjects: Agonists; Amino Acid Sequence; Amino Acid Substitution; Animals; Antibodies, Monoclonal - immunology; Biological and medical sciences; Cell adhesion; Cell Line; Cell physiology; CHO cells; Cytometry; Fibronectins - metabolism; Fundamental and applied biological sciences. Psychology; Humans; Integrin beta Chains - chemistry; Integrin beta Chains - metabolism; Integrin beta1 - chemistry; Integrin beta1 - metabolism; Integrin beta3 - chemistry; Integrin beta3 - metabolism; Integrins; Ligands; Molecular and cellular biology; Molecular Sequence Data; Mutation; NIH 3T3 cells; Physiological aspects; Physiological regulation; Plasmids; Platelet Glycoprotein GPIIb-IIIa Complex - chemistry; Platelet Glycoprotein GPIIb-IIIa Complex - immunology; Platelet Glycoprotein GPIIb-IIIa Complex - metabolism; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Recombinant Proteins - metabolism; RNA, Small Interfering; Signal Transduction; Stem cells; Talin - metabolism; Transfection; United States; Binding; Signal transduction; Integrin; Cell line; Ligand; Molecular interaction; Adhesion
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1086652

Control of integrin affinity for ligands (integrin activation) is essential for normal cell adhesion, migration, and assembly of an extracellular matrix. Integrin activation is usually mediated through the integrin β subunit cytoplasmic tail and can be regulated by many different biochemical signaling pathways. We report that specific binding of the cytoskeletal protein talin to integrin β subunit cytoplasmic tails leads to the conformational rearrangements of integrin extracellular domains that increase their affinity. Thus, regulated binding of talin to integrin β tails is a final common element of cellular signaling cascades that control integrin activation.