Microsatellite primers for red drum

• Published in: Fishery bulletin (Washington, D.C.) Vol. 106; no. 4; pp. 476 - 482
• Main Authors: Karlsson, Sten, Renshaw, Mark A, Rexroad, III, Caird E, Gold, John R
• Format: Journal Article
• Language: English
• Published: National Marine Fisheries Service 01.10.2008
• Subjects: Aquaculture; Channel bass; Fish-culture; Methods; Observations; Sciaenops ocellatus; Technology application; Tracking and trailing; United States; ASW, USA, Texas; ASW, USA, Texas, Galveston Bay
• ISSN: 0090-0656

In this note, we document polymerase-chain-reaction (PCR) primer pairs for 101 nuclear-encoded microsatel-lites designed and developed from a genomic library for red drum (Sciaenops ocellatus). Details of the genomic library construction, the sequencing of positive clones, primer design, and PCR protocols may be found in Karlsson et al. (2008). The 101 microsatel-lites (Genbank Accession Numbers EU015882-EU015982) were amplified successfully and used to genotype 24 red drum obtained from Galveston Bay, Texas. A total of 69 of the microsatellites had an uninterrupted (perfect) dinucleotide motif, and 30 had an imperfect dinucleotide motif; one microsatellite had an imperfect tetranucleotide motif, and one had an imperfect and compound motif. Sizes of the cloned alleles ranged from 84 to 252 base pairs. A 'blast' search of the Genbank database indicated that all of the primers and the cloned alleles were unique (i.e., not duplicated).