Evaluation of the effects of omega-3 & interferon alpha-2b administration on partial bladder outlet obstruction in a rat model

• Published in: Indian journal of medical research (New Delhi, India : 1994) Vol. 144; no. 4; pp. 572 - 579
• Main Authors: Firat, Fatih, Uluocak, Nihat, Erdemir, Fikret, Atilgan, Dogan, Markoc, Fatma, Parlaktas, Bekir Suha, Yasar, Adem
• Format: Journal Article
• Language: English
• Published: India Medknow Publications and Media Pvt. Ltd 01.10.2016; Scientific Scholar; Medknow Publications & Media Pvt Ltd
• Subjects: Animals; Antioxidants; Bladder; Cardiovascular disease; Care and treatment; Catheters; Collagen; Disease Models, Animal; Fatty acids; Fatty Acids, Omega-3 - administration & dosage; Fibroblasts; Fibrosis - drug therapy; Fibrosis - pathology; Free radicals; Growth factors; Humans; Inflammation; Inflammation - drug therapy; Inflammation - pathology; Interferon alfa-2b; Interferon-alpha - administration & dosage; Male; Nitrates; Omega 3 fatty acids; Original; Oxidative stress; Oxidative Stress - drug effects; Physiological aspects; Proteins; Rats; Recombinant Proteins - administration & dosage; Ureteral obstruction; Urinary Bladder - drug effects; Urinary Bladder - pathology; Urinary Bladder Neck Obstruction - drug therapy; Urinary Bladder Neck Obstruction - pathology; Urogenital system
• ISSN: 0971-5916
• DOI: 10.4103/0971-5916.200899

In bladder outlet obstruction-induced rat models, the transforming growth factor-beta (TGF-β) and collagen ratios have been shown to be increased. Increased TGF-β leads to fibrosis. In this study, the effect of omega-3 and interferon alpha-2b (IFN α-2b) was investigated on oxidative stress, inflammation and fibrosis in bladder structure in a partial bladder outlet obstruction (PBOO) rat model. A total of 35 male Wistar albino rats, weighing 300-350 g, were used in the study. The rats were randomly divided into five groups. At the end of the experimental period, bladders were harvested from all the rats, and pathological analysis of the rat bladder tissues was performed. In addition, investigations were carried out with enzymatic and non-enzymatic antioxidant systems to study the antioxidant properties of omega-3 fatty acid and IFN alpha-2b. Increased bladder weight in the PBOO group, in comparison to the control group, was decreased by the administration of omega-3 and IFN α-2b (P=0.002). Significantly higher superoxide dismutase (SOD) levels were detected in group 2 in comparison to the control group. It was also detected that serum SOD, glutathione peroxidase and nitric oxide (NO) levels were significantly higher in group 2 when compared to the control group (P<0.05). In the pathologic evaluation, group 2 showed significantly increased inflammation and fibrosis compared to the control group. Omega-3 treatment significantly decreased inflammation. It was shown that IFN α-2b application partially decreased inflammation. The results of the present study showed that in addition to the standard primary approaches to prevent the damage to the upper urinary tract secondary to PBOO, omega-3 fatty acid and IFN α-2b could be beneficial as adjunct treatment in clinical practice. However, this needs to be further investigated with prospective, randomized clinical trials with larger sample sizes.