In Situ Proximity Ligation Assay to Detect the Interaction Between Plant Transcription Factors and Other Regulatory Proteins

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1830; p. 325
• Main Authors: Xu, Yifeng, Gan, Eng-Seng, Ito, Toshiro
• Format: Journal Article
• Language: English
• Published: United States 2018
• Subjects: Protein-protein interaction; Tissues; Transcription factor; Nuclei; In situ proximity ligation assay
• ISSN: 1940-6029
• DOI: 10.1007/978-1-4939-8657-6_19

In plants, a lot of transcription factors fulfill their roles in gene regulation through the interaction with other regulatory proteins and co-factors. Thus, confirmation of protein-protein interaction is key to understand the precise function of transcription factors. Many methods have been developed to investigate the protein-protein interaction in vivo and in vitro. In situ Proximity Ligation Assay (PLA) is an innovative method to test protein-protein interaction in your tissues or cells of interest in vivo. Furthermore, this method allows us to detect transient interaction and low-abundance protein interaction with single molecule resolution. In this chapter, we describe a detailed protocol for the study of interaction between plant transcription factors and other regulatory proteins, in the scale of single nuclei of plant organ, tissues and cells.