Characterization of restriction endonuclease AjoI from Acinetobacter johnsonii

A new type II restriction endonuclease, named AjoI, was detected in Acinetobacter johnsonii. The enzyme AjoI, an isoschizomer of PstI, recognized the hexanucleotide sequence [5′‐CTGCA/G‐3′], with a cleavage site generating fragments of DNA with protruding cohesive 3′ termini.

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Bibliographic Details
Published inFEMS microbiology letters Vol. 117; no. 1; pp. 97 - 102
Main Authors Nowak, A., Kur, J., Gospodarek, E., Bielawski, K.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 15.03.1994
Blackwell
Subjects
Acinetobacter - enzymology
Acinetobacter johnsonii
Bacteriology
Base Sequence
Binding Sites
Biological and medical sciences
Deoxyribonucleases, Type II Site-Specific - isolation & purification
Deoxyribonucleases, Type II Site-Specific - metabolism
DNA - genetics
DNA - metabolism
Fundamental and applied biological sciences. Psychology
Isoschizomer
Metabolism. Enzymes
Microbiology
Molecular Sequence Data
PstI endonuclease
Recognition sequence
Restriction endonuclease
Substrate Specificity
Sequence specificity
Enzymatic activity
Enzyme
Cleavage site
Neisseriaceae
Restriction endonuclease
Bacteria
Hydrolases
Micrococcales
Esterases
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Summary:A new type II restriction endonuclease, named AjoI, was detected in Acinetobacter johnsonii. The enzyme AjoI, an isoschizomer of PstI, recognized the hexanucleotide sequence [5′‐CTGCA/G‐3′], with a cleavage site generating fragments of DNA with protruding cohesive 3′ termini.
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ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1994.tb06748.x