Qualitative and Quantitative Estimation of Bacterial Lipid Production

An ever increasing energy demand and fast depletion of fossil fuels have led to increased consideration of bacterial lipids as a renewable biofuel source. Many methods are available for both physical and chemical extraction of bacterial lipids. The method of choice will depend on the nature of sampl...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1995; p. 91
Main Authors Muralitharan, Gangatharan, Gayathri, Manickam, Shunmugam, Sumathy
Format Journal Article
LanguageEnglish
Published United States 01.01.2019
Subjects
Bacteria - chemistry
Bacteria - growth & development
Bacteria - metabolism
Biofuels - analysis
Biomass
Chromatography, Gas - methods
Cyanobacteria - chemistry
Cyanobacteria - growth & development
Cyanobacteria - metabolism
Esterification
Fatty Acids - analysis
Fatty Acids - metabolism
Lipids - analysis
Lipids - isolation & purification
Lipogenesis
Lipid extraction
Bacteria
Fatty acid methyl ester (FAME)
Transesterification
Growth kinetic parameters
Cyanobacteria
Online AccessGet more information

Cover

More Information
Summary:An ever increasing energy demand and fast depletion of fossil fuels have led to increased consideration of bacterial lipids as a renewable biofuel source. Many methods are available for both physical and chemical extraction of bacterial lipids. The method of choice will depend on the nature of sample to be analyzed, combinations of solvent systems preferred, content and quality of the lipid to be analyzed, types of equipment available, and time of the extraction procedures employed. Here we describe the most reliable, routine method of extracting bacterial lipids and evaluating the growth kinetic parameters like biomass and lipid productivity and lipid content. We also describe the method of comparing bacterial fatty acid methyl ester peaks with standard peaks for analysis.
ISSN:1940-6029
DOI:10.1007/978-1-4939-9484-7_4