Direct and Continuous Measurement of Phospholipase D Activities Using the Chelation-Enhanced Fluorescence Property of 8-Hydroxyquinoline

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1835; p. 129
• Main Authors: Rahier, Renaud, Abla, Houda, Arhab, Yani, Noiriel, Alexandre, Abousalham, Abdelkarim
• Format: Journal Article
• Language: English
• Published: United States 01.01.2018
• Subjects: Enzyme Activation; Enzyme Assays - methods; Enzyme Assays - standards; Fluorescent Dyes; Humans; Hydrolysis; Kinetics; Oxyquinoline - metabolism; Phospholipase D - chemistry; Phospholipase D - metabolism; 8-Hydroxyquinoline; Assay; Screening; Inhibitors; Fluorescence; Microplate; Transphosphatidylation; Phospholipase D; Phosphatidic acid

Phospholipase D (PLD) hydrolyzes phospholipids to form phosphatidic acid (PA) and the corresponding headgroup. To date, PLD has been linked to several pathologies, such as cancer, making this enzyme an important therapeutic target. However, most PLD assays developed so far are either discontinuous o...