Quantifying small molecules by mass spectrometry

If the analysis of a series of standards is shown to be reproducible and rugged under well-defined conditions, a linear range and the relationship between the response and the concentration can be defined. [...]the response for each unknown can be translated into a concentration or amount by referri...

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Bibliographic Details
Published inLC GC North America Vol. 33; no. 1; p. 34
Main Authors Gale, P. Jane, Yergey, Alfred L, Duncan, Mark W, Yu, Kate
Format Magazine Article Trade Publication Article
LanguageEnglish
Published Monmouth Junction UBM LLC 01.01.2015
MultiMedia Healthcare Inc
Subjects
Accuracy
Calibration
Chromatography
Confidence intervals
Gas detectors
Mass spectrometry
Quantitative analysis
Scientific imaging
Spectrometers
Technology application
United States
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Summary:If the analysis of a series of standards is shown to be reproducible and rugged under well-defined conditions, a linear range and the relationship between the response and the concentration can be defined. [...]the response for each unknown can be translated into a concentration or amount by referring to that relationship (or calibration curve). To ensure that the uncertainty associated with the results has been acknowledged and accepted, it is often useful, at the outset of planned work, to do as follows: * Define the analytical problem, and consider the needs of all interested parties. * Evaluate the perceived risk associated with the analyte and the consequences associated with the result. * Balance thoroughness with time and resources. * Define the tolerance or uncertainty. * Specify limits for accuracy, precision, and false identification. * Estimate the expected concentrations, and choose the range of calibrators accordingly.
ISSN:1527-5949
1939-1889