New approach to model effects of darkness, melatonin and serotonin on Tetrahymena thermophila growth and production of hydrolytic enzymes

Central composite design was used to quantify the relationship between darkness, melatonin and serotonin with growth of Tetrahymena thermophila and hydrolytic enzyme activities. Circadian variation was the most important factor to optimise the maximal population without a long generation time; i.e....

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Bibliographic Details
Published inBiotechnology letters Vol. 24; no. 10; pp. 769 - 774
Main Authors LECLERCQ, B, EXBRAYAT, J. M, DUYME, F, DE CONINCK, J
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 02.05.2002
Springer Nature B.V
Subjects
Biological and medical sciences
Biotechnology
Enzymatic activity
Enzymes
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Serotonin
Tetrahymena thermophila
Protozoa
Darkness
Melatonin
Tetrahymena thermophila
Enzymatic activity
Serotonin
Enzyme
Production
Microorganism culture
Hydrolases
Ciliata
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Summary:Central composite design was used to quantify the relationship between darkness, melatonin and serotonin with growth of Tetrahymena thermophila and hydrolytic enzyme activities. Circadian variation was the most important factor to optimise the maximal population without a long generation time; i.e. light at the beginning of culture for 640 min followed by darkness for 800 min. In addition, the medium should be supplemented with serotonin and melatonin (0.1 mM each). Supplemental melatonin and serotonin increased lipase, phosphatase and protease activities but without a circadian variation.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1015515918815