Accelerated Tissue Processing With Minimal Formalin Fixation Time for 9-Gauge Vacuum-Assisted Breast Biopsy Specimens: A Proof of Principle

Objectives: Vacuum-assisted biopsy (VAB) of the breast seems unsuitable for rapid processing due to large size. We tested microwave-based acceleration. Methods: As a proof-of-principle study, 9-gauge VAB specimens were taken from eight mastectomy specimens. Forty-two biopsy specimens were processed....

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Bibliographic Details
Published inAmerican journal of clinical pathology Vol. 153; no. 1; pp. 58 - 65
Main Authors Bulte, Joris P, Halilovic, Altuna, Burgers, Lambert J.M, Diepenbroek, Coos J.M, de la Roij, Robin A.K, Mann, Ritse M, van der Leest, Marloes, van Cleef, Patricia H.J, Strobbe, Luc J.A, de Wilt, Johannes H.W, Bult, Peter
Format Journal Article
LanguageEnglish
Published Chicago Oxford University Press 01.01.2020
Subjects
Biopsy
Breast
Breast cancer
Diagnosis
E-cadherin
Epidermal growth factor
ErbB-2 protein
Estrogen receptors
Fluorescence in situ hybridization
Immunohistochemistry
Medical diagnosis
Original
Progesterone
Quality
Vacuum
United States
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Summary:Objectives: Vacuum-assisted biopsy (VAB) of the breast seems unsuitable for rapid processing due to large size. We tested microwave-based acceleration. Methods: As a proof-of-principle study, 9-gauge VAB specimens were taken from eight mastectomy specimens. Forty-two biopsy specimens were processed. Quality of H&E was evaluated in 84 slides, and estrogen receptor (ER), progesterone receptor (PR), E-cadherin, and human epidermal growth factor receptor 2 (HER2) stains were evaluated in six slides. Preoperative biopsy specimens were used as a control. Results: Diagnostic quality of H&E slides was good in 87%, reasonable in 12%, and low in 1%o. Quality of E-cadherin was good in 75%o and reasonable in 25%. Quality of ER was good in 83% and reasonable in 17%o. PR and both HER2 immunohistochemistry and fluorescence in situ hybridization were good in all slides. Quality of experimental slides was similar to control slides. Conclusions: Nine-gauge VAB specimens can be processed within 4 hours. Slides are suitable for all routine pathologic stains. This enables a same-day diagnosis. Key Words: Breast cancer; Diagnosis; Fixation; Accelerated processing; Core needle biopsy
ISSN:0002-9173
1943-7722
DOI:10.1093/AJCP/AQZ111