Proanthocyanin‐Capped Biogenic TiO2 Nanoparticles with Enhanced Penetration, Antibacterial and ROS Mediated Inhibition of Bacteria Proliferation and Biofilm Formation: A Comparative Approach

• Published in: Chemistry : a European journal Vol. 27; no. 18; pp. 5817 - 5829
• Main Authors: Alomary, Mohammad N., Ansari, Mohammad Azam
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley Subscription Services, Inc 26.03.2021
• Subjects: antibiotics; Antiinfectives and antibacterials; Biofilms; Cell proliferation; Chemistry; Dispersion; Fourier analysis; Inductively coupled plasma mass spectrometry; Infrared analysis; Internalization; Intracellular; Mass spectroscopy; Nanoparticles; Nucleation; Penetration; Planktonic cells; Plant extracts; Polyphenols; Pseudomonas aeruginosa; Reactive oxygen species; Seeds; Spectral analysis; Spectrum analysis; Stability; Strains (organisms); Synthesis; titanium; Titanium dioxide; Urinary tract; Urinary tract infections
• ISSN: 0947-6539; 1521-3765
• DOI: 10.1002/chem.202004828

Biofunctionalized TiO2 nanoparticles with a size range of 18.42±1.3 nm were synthesized in a single‐step approach employing Grape seed extract (GSE) proanthocyanin (PAC) polyphenols. The effect of PACs rich GSE corona was examined with respect to 1) the stability and dispersity of as‐synthesized GSE‐TiO2‐NPs, 2) their antiproliferative and antibiofilm efficacy, and 3) their propensity for internalization and reactive oxygen species (ROS) generation in urinary tract infections (UTIs) causing Gram‐negative Pseudomonas aeruginosa and Gram‐positive Staphylococcus saprophyticus strains. State‐of‐the‐art techniques were used to validate GSE‐TiO2‐NPs formation. Comparative Fourier transformed infrared (FTIR) spectral analysis demonstrated that PACs linked functional ‐OH groups likely play a central role in Ti4+ reduction and nucleation to GSE‐TiO2‐NPs, while forming a thin, soft corona around nascent NPs to attribute significantly enhanced stability and dispersity. Transmission electron microscopic (TEM) and inductively coupled plasma mass‐spectroscopy (ICP‐MS) analyses confirmed there was significantly (p<0.05) enhanced intracellular uptake of GSE‐TiO2‐NPs in both Gram‐negative and ‐positive test uropathogens as compared to bare TiO2‐NPs. Correspondingly, compared to bare NPs, GSE‐TiO2‐NPs induced intracellular ROS formation that corresponded well with dose‐dependent inhibitory patterns of cell proliferation and biofilm formation in both the tested strains. Overall, this study demonstrates that ‐OH rich PACs of GSE corona on biogenic TiO2‐NPs maximized the functional stability, dispersity and propensity of penetration into planktonic cells and biofilm matrices. Such unique merits warrant the use of GSE‐TiO2‐NPs as a novel, functionally stable and efficient antibacterial nano‐formulation to combat the menace of UTIs in clinical settings. All in the delivery: Biofunctionalized TiO2 nanoparticles were synthesized in a single‐step from Grape seed extract proanthocyanin polyphenols. The GSE‐TiO2‐NPs can be used as functionally stable antibacterial agents to combat UTIs in clinical settings.