Adherence of type 1-fimbriated Escherichia coli to uroepithelial cells: More in diabetic women than in control subjects

• Published in: Diabetes care Vol. 25; no. 8; pp. 1405 - 1409
• Main Authors: GEERLINGS, Suzanne E, MEILAND, Ruby, VAN LITH, Emiel C, BROUWER, Ellen C, GAASTRA, Wim, HOEPELMAN, Andy I. M
• Format: Journal Article
• Language: English
• Published: Alexandria, VA American Diabetes Association 01.08.2002
• Subjects: Adult; Associated diseases and complications; Bacteria; Bacterial Adhesion; Bacteriuria - complications; Bacteriuria - microbiology; Biological and medical sciences; Cell Line; Diabetes; Diabetes Mellitus, Type 1 - microbiology; Diabetes. Impaired glucose tolerance; Diabetics; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Escherichia coli - metabolism; Escherichia coli - pathogenicity; Escherichia coli infections; Escherichia coli Infections - complications; Excretory system; Female; Fimbriae, Bacterial - metabolism; Health aspects; Humans; Lewis Blood-Group System; Medical sciences; Middle Aged; Risk factors; Urinary Bladder - cytology; Urinary tract infections; Urothelium - cytology; Urothelium - microbiology; Virulence; Women; Netherlands; Endocrinopathy; Human; Cell culture; Urinary system disease; Bacteriuria; Healthy subject; Pathogenesis; Diabetes mellitus; Escherichia coli; Adhesion; In vitro; Infection; Urinary bladder; Bacteriosis; Bacteria; Female; Comparative study; Enterobacteriaceae
• ISSN: 0149-5992; 1935-5548
• DOI: 10.2337/diacare.25.8.1405

Women with diabetes have bacteriuria more often than women without diabetes. Because Escherichia coli adhere better to vaginal cells of nondiabetic patients with recurrent urinary tract infections (UTIs) than to those obtained from healthy control subjects, it was hypothesized that E. coli adhere more to the uroepithelial cells of diabetic women, either because of substances excreted in the urine (e.g., albumin, glucose, and Tamm Horsfall protein) or because of a difference in the uroepithelial cells. A T24 bladder cell line and uroepithelial cells of 25 diabetic women and 19 control subjects were incubated with three different E. coli strains. The mean numbers of type 1-fimbriated E. coli that adhered to diabetic and control cells were 12.9 and 6.1 (P = 0.001), respectively, whereas those of P-fimbriated E. coli were 8.8 and 8.1 (P = 0.8), and those of nonfimbriated E. coli were 2.7 and 3.4 (P = 0.4). The addition of various substances did not influence the adherence of E. coli to a T24 bladder cell line. Type 1-fimbriated E. coli adhere more to diabetic than to control uroepithelial cells.