Characterization of TrypZean: a plant-based alternative to bovine-derived trypsin

Recombinant bovine trypsin has been produced in transgenic maize as a pathogen-free alternative to the animal-derived reagent. Biological reagents intended for pharmaceutical purposes require comprehensive analysis including detailed information about sequence and posttranslational modifications. In...

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Bibliographic Details
Published inBiopharm International Vol. 24; no. 10; p. 44
Main Authors Ray, Kevin, Jalili, Pegah R
Format Magazine Article Trade Publication Article
LanguageEnglish
Published Monmouth Junction MJH Life Sciences Media 01.10.2011
MultiMedia Healthcare Inc
Subjects
Amino acids
Biotechnology
Enzymes
Food
Genetically engineered foods
Mass spectrometry
Molecular weight
Peptides
Pharmaceutical industry
Pharmacology
Proteases
Proteins
Scientific imaging
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Summary:Recombinant bovine trypsin has been produced in transgenic maize as a pathogen-free alternative to the animal-derived reagent. Biological reagents intended for pharmaceutical purposes require comprehensive analysis including detailed information about sequence and posttranslational modifications. In this study, several techniques, including mass spectroscopy (MS) analysis of the intact protein, peptide mapping, and MS analysis of released glycans were applied and an in-depth characterization of maize derived trypsin was achieved, revealing an unusual nonconsensus N-linked glycosylation. [PUBLICATION ABSTRACT]
ISSN:1542-166X
1939-1862