The Use of a Semi-Automated System to Measure Mouse Natural Killer T (NKT) Cell Activation by Lipid-Loaded Dendritic Cells

Cell-based assays are widely used in all aspects of research ranging from understanding basic biological function to identifying compounds for disease intervention. Immortalized cell lines have been ideal components of these assays due to the low cost of growth, easy maintenance, and the ability to...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1592; p. 249
Main Authors Ghumra, Ashfaq, Alcocer, Marcos
Format Journal Article
LanguageEnglish
Published United States 2017
Subjects
Animals
Cell Line
Dendritic Cells - immunology
Interleukin-2 - immunology
Lipids - immunology
Lymphocyte Activation - immunology
Mice
Natural Killer T-Cells - immunology
Natural Killer T (NKT) cells
Cell-based assays
Biomek® 3000
Robotics
ELISA
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Summary:Cell-based assays are widely used in all aspects of research ranging from understanding basic biological function to identifying compounds for disease intervention. Immortalized cell lines have been ideal components of these assays due to the low cost of growth, easy maintenance, and the ability to obtain homogenous cell populations. Like other molecular assays, cell-based systems have been automated to reduce experimental error. Complex lipids are now recognized as important components of the allergic response, the study of the interaction between NKTs and lipid-activated DCs opens a new perspective into the intrinsic allergenicity of proteins. Here, we describe a semi-automated method to measure IL-2 release upon activation of mouse NKT cells (DN32.D3) by various lipids in a 384-well plate using the Biomek 3000 laboratory automated workstation (Beckman Coulter).
ISSN:1940-6029
DOI:10.1007/978-1-4939-6925-8_19