Analysis of Angiotensin Metabolism in the Kidney Using Mass Spectrometry

The renin angiotensin system (RAS) is a highly complex enzymatic system consisting of multiple peptide hormones, enzymes, and receptors. A thorough characterization of angiotensin peptide metabolism is crucial for understanding pathological states associated with an imbalanced RAS. Here, we describe...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1614; p. 189
Main Authors Grobe, Nadja, Elased, Khalid M
Format Journal Article
LanguageEnglish
Published United States 01.01.2017
Subjects
Angiotensins - metabolism
Animals
Kidney - metabolism
Mice
Peptide Fragments - metabolism
Renin-Angiotensin System - physiology
Software
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
ACE2
ACE
Enzyme activity
Imaging
MALDI
Angiotensin metabolism
NEP
Renin-angiotensin system
Mass spectrometry
Kidney
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Summary:The renin angiotensin system (RAS) is a highly complex enzymatic system consisting of multiple peptide hormones, enzymes, and receptors. A thorough characterization of angiotensin peptide metabolism is crucial for understanding pathological states associated with an imbalanced RAS. Here, we describe two matrix-assisted laser desorption/ionization (MALDI) mass spectrometric (MS) approaches for the assessment of in vitro and in situ RAS enzymatic activities in the kidney using the natural angiotensin peptide substrates. These MS techniques demonstrate high specificity and are superior over conventional spectrophotometric or colorimetric assays since multiple proteolytic cleavage sites can be detected, thus unraveling the complexity of the RAS.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7030-8_15