Development of Dual Quantitative Lateral Flow Immunoassay for the Detection of Mycotoxins

Lateral flow immunoassays have been widely used in recent years for detection of toxins, heavy metals, and biomarkers. To improve the efficiency of individual lateral flow immunoassays, multiplex analytical strips play an important role in the detection of several important analytes. In this chapter...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1571; p. 435
Main Authors Wang, Yuan-Kai, Yan, Ya-Xian, Sun, Jian-He
Format Journal Article
LanguageEnglish
Published United States 01.01.2017
Subjects
Antibodies, Monoclonal
Calibration
Colloids
Gold
Immunoassay - instrumentation
Immunoassay - methods
Immunoconjugates
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
Mycotoxins - analysis
Point-of-Care Systems
Workflow
Aflatoxin B1
Fumonisin B1
Deoxynivalenol
Lateral flow
Gold nanoparticles
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Summary:Lateral flow immunoassays have been widely used in recent years for detection of toxins, heavy metals, and biomarkers. To improve the efficiency of individual lateral flow immunoassays, multiplex analytical strips play an important role in the detection of several important analytes. In this chapter, development of a dual lateral flow immunoassay is presented for detection of a variety of low molecular weight molecules. Various buffers, additives, and materials are introduced and evaluated. Depending on the analyte to be tested, the technique allows for selection of optimum buffers, additives, and other materials.
ISSN:1940-6029
DOI:10.1007/978-1-4939-6848-0_26