In Vitro Suppression of CD4 + T-Cell Responses by Murine and Human Myeloid-Derived Suppressor Cells

Myeloid-derived suppressor cells (MDSCs) are myeloid precursors of macrophages, dendritic cells, and neutrophils with a prominent role in the regulation of immune responses in cancer, infection, and autoimmunity. Herein, we describe a protocol for the isolation of murine and human MDSCs and the asse...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1899; p. 119
Main Authors Vlachou, Katerina, Verginis, Panayotis
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
CD4+ T-cell isolation
In vitro proliferation assay
Cell sorting
Carboxyfluorescein succinimidyl ester (CFSE)
Myeloid-derived suppressor cells
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Summary:Myeloid-derived suppressor cells (MDSCs) are myeloid precursors of macrophages, dendritic cells, and neutrophils with a prominent role in the regulation of immune responses in cancer, infection, and autoimmunity. Herein, we describe a protocol for the isolation of murine and human MDSCs and the assessment of their ability to suppress CD4 T-cell responses in vitro.
ISSN:1940-6029
DOI:10.1007/978-1-4939-8938-6_9