High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1795; p. 49
• Main Authors: Halder, Vivek, Kombrink, Erich
• Format: Journal Article
• Language: English
• Published: United States 01.01.2018
• Subjects: Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis - metabolism; Enzyme Assays; Gene Expression Regulation, Plant - drug effects; Genes, Reporter; Glucuronidase - genetics; High-Throughput Screening Assays; Plant Growth Regulators - chemistry; Plant Growth Regulators - pharmacology; Plants, Genetically Modified; Salicylic Acid - metabolism; Signal Transduction - drug effects; Small Molecule Libraries; Arabidopsis thaliana; Glucuronidase activity; Salicylic acid; Chemical screen; Reporter gene expression; Chemical genetics; Phytohormone signaling; Plant defense
• ISSN: 1940-6029
• DOI: 10.1007/978-1-4939-7874-8_5

Salicylic acid (SA) is a vital phytohormone that is intimately involved in coordination of the complex plant defense response to pathogen attack. Many aspects of SA signaling have been unraveled by classical genetic and biochemical methods using the model plant Arabidopsis thaliana, but many details remain unknown, owing to the inherent limitations of these methods. In recent years, chemical genetics has emerged as an alternative scientific strategy to complement classical genetics by virtue of identifying bioactive chemicals or probes that act selectively on their protein targets causing either activation or inhibition. Such selective tools have the potential to create conditional and reversible chemical mutant phenotypes that may be combined with genetic mutants. Here, we describe a facile chemical screening methodology for intact Arabidopsis seedlings harboring the β-glucuronidase (GUS) reporter by directly quantifying GUS activity in situ with 4-methylumbelliferyl-β-D-glucuronide (4-MUG) as substrate. The quantitative nature of this screening assay has an obvious advantage over the also convenient histochemical GUS staining method, as it allows application of statistical procedures and unbiased hit selection based on threshold values as well as distinction between compounds with strong or weak bioactivity. We show pilot screens for chemical activators or inhibitors of salicylic acid-mediated defense signaling using the Arabidopsis line expressing the SA-inducible PR1p::GUS reporter gene. Importantly, the screening methodology provided here can be adopted for any inducible GUS reporter line.