Nestin-Based Reporter Transgenic Mouse Lines

Nestin expression marks stem and progenitor cells of the neural lineage. Transgenic mouse lines, originally generated to identify neural stem cells, can also help to identify, track, and isolate stem and progenitor cells in a range of tissues of the ectodermal, endodermal, and mesodermal origin. Her...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1453; p. 7
Main Authors Mignone, John, Peunova, Natalia, Enikolopov, Grigori
Format Journal Article
LanguageEnglish
Published United States 01.01.2016
Subjects
Animals
Gene Expression
Genes, Reporter
Genotype
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Immunohistochemistry
Mice
Mice, Transgenic
Nestin - genetics
Nestin - metabolism
Recombinant Fusion Proteins
Stem Cells - metabolism
Reporter lines
Nestin
Hair-follicle-associated pluripotent (HAP)
Immunocytochemistry
Transgenic animals
Stem cells
Nestin-GFP
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Summary:Nestin expression marks stem and progenitor cells of the neural lineage. Transgenic mouse lines, originally generated to identify neural stem cells, can also help to identify, track, and isolate stem and progenitor cells in a range of tissues of the ectodermal, endodermal, and mesodermal origin. Here, we describe the generation of transgenic mouse lines expressing fluorescent proteins (FP) under the control of critical regulatory elements of the nestin gene and their use for identifying and analyzing adult stem and progenitor cells in various tissues.
ISSN:1940-6029
DOI:10.1007/978-1-4939-3786-8_2