In-Vitro Dissolution Dynamic Nuclear Polarization for Sensitivity Enhancement of NMR with Biological Molecules

Dissolution dynamic nuclear polarization (D-DNP) is a technique to prepare hyperpolarized nuclear spin states, yielding a signal enhancement of several orders of magnitude for liquid-state NMR. Here, we describe experimental procedures for the application of D-DNP in high-resolution NMR of biochemic...

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Published inMethods in molecular biology (Clifton, N.J.) Vol. 1688; p. 155
Main Authors Kim, Yaewon, Wang, Yunyi, Chen, Hsueh-Ying, Hilty, Christian
Format Journal Article
LanguageEnglish
Published United States 01.01.2018
Subjects
Kinetics
Nuclear Magnetic Resonance, Biomolecular - methods
Real-time NMR
Spin correlations
Kinetics
Spin relaxation
Hyperpolarization
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Summary:Dissolution dynamic nuclear polarization (D-DNP) is a technique to prepare hyperpolarized nuclear spin states, yielding a signal enhancement of several orders of magnitude for liquid-state NMR. Here, we describe experimental procedures for the application of D-DNP in high-resolution NMR of biochemical compounds, to determine the time evolution of biochemical processes and intermolecular interactions.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7386-6_8