Osteogenic Differentiation from Embryonic Stem Cells

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1341; p. 425
• Main Authors: Yu, Yanhong, Pilquil, Carlos, Opas, Michal
• Format: Journal Article
• Language: English
• Published: United States 01.01.2016
• Subjects: Animals; Cell Culture Techniques - methods; Cell Differentiation; Cell Proliferation; Embryoid Bodies - cytology; Embryonic Stem Cells - cytology; Mice; Osteogenesis; Tissue Culture Techniques - methods; Murine embryonic stem cells; Embryoid bodies; Hanging drop; Osteogenesis; Routine culture

Embryonic stem (ES) cells have been widely studied due to their pluripotency and their potential of self-renewal. Murine ES cells are useful in investigating the molecular pathways underlying their differentiation to various mature cell types in the body. This chapter describes the maintenance of mu...