Osteogenic Differentiation from Embryonic Stem Cells

Embryonic stem (ES) cells have been widely studied due to their pluripotency and their potential of self-renewal. Murine ES cells are useful in investigating the molecular pathways underlying their differentiation to various mature cell types in the body. This chapter describes the maintenance of mu...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1341; p. 425
Main Authors Yu, Yanhong, Pilquil, Carlos, Opas, Michal
Format Journal Article
LanguageEnglish
Published United States 01.01.2016
Subjects
Animals
Cell Culture Techniques - methods
Cell Differentiation
Cell Proliferation
Embryoid Bodies - cytology
Embryonic Stem Cells - cytology
Mice
Osteogenesis
Tissue Culture Techniques - methods
Murine embryonic stem cells
Embryoid bodies
Hanging drop
Osteogenesis
Routine culture
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Summary:Embryonic stem (ES) cells have been widely studied due to their pluripotency and their potential of self-renewal. Murine ES cells are useful in investigating the molecular pathways underlying their differentiation to various mature cell types in the body. This chapter describes the maintenance of murine ES cells in culture and a routine ES cell osteogenic differentiation protocol utilized in our laboratory.
ISSN:1940-6029
DOI:10.1007/7651_2014_126