Differentiation of Adipocytes in Monolayer from Mouse Embryonic Stem Cells

Obesity and its comorbidity incidence have increased worldwide during the past 10 years. In consequence, researchers have drawn their attention to the understanding of adipocyte differentiation. Several cellular model systems have been established; however no efficient protocol could be developed so...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1341; p. 407
Main Authors Cuaranta-Monroy, Ixchelt, Simandi, Zoltan, Nagy, Laszlo
Format Journal Article
LanguageEnglish
Published United States 01.01.2016
Subjects
Adipocytes - cytology
Adipogenesis
Animals
Cell Culture Techniques - methods
Cell Line
Mice
Mouse Embryonic Stem Cells - cytology
Ascorbic acid
Adipocyte differentiation
Monolayer culture
Mouse embryonic stem cell
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Summary:Obesity and its comorbidity incidence have increased worldwide during the past 10 years. In consequence, researchers have drawn their attention to the understanding of adipocyte differentiation. Several cellular model systems have been established; however no efficient protocol could be developed so far to differentiate the pluripotent embryonic stem cells to adipocytes. In this chapter, we describe a detailed protocol that is optimized for mouse embryonic stem cells. The result of this differentiation is a homogenous adipocyte monolayer culture that can be used for several applications including developmental and pharmacological research.
ISSN:1940-6029
DOI:10.1007/7651_2015_219