A direct and simple method for rapidly counting viable chains of Leuconostoc in batch cultures

Plating determines the concentration in viable cells of bacteria but remains time-consuming and inaccurate. A direct and simple method combining two techniques, haemacytometry and epifluorescence, gives both the viability and the concentration in viable cells. The method was evaluated using Leuconos...

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Bibliographic Details
Published inBiotechnology techniques Vol. 9; no. 6; pp. 451 - 456
Main Authors PLIHON, F, TAILLANDIER, P, STREHAIANO, P
Format Journal Article
LanguageEnglish
Published London Chapman and Hall 01.06.1995
Springer Verlag
Subjects
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Biotechnology
Chemical and Process Engineering
Chemical engineering
Chemical Sciences
Engineering Sciences
Fundamental and applied biological sciences. Psychology
Leuconostoc mesenteroides
Methods. Procedures. Technologies
Microbiology
Others
Various methods and equipments
Performance evaluation
Leuconostoc mesenteroides
Lactic acid bacteria
Enumeration
Fluorescence
Method
Streptococcaceae
Cytometry
Batchwise
Microorganism culture
Bacteria
Micrococcales
Viability
Comparative study
Viable cell
Batch culture
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Summary:Plating determines the concentration in viable cells of bacteria but remains time-consuming and inaccurate. A direct and simple method combining two techniques, haemacytometry and epifluorescence, gives both the viability and the concentration in viable cells. The method was evaluated using Leuconostoc mesenteroides.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00160835