Fresh and aged human lymphocyte metaphase slides are equally usable for GTG banding

The identification of chromosomes for routine cytogenetic analysis is based on quality of metaphases and good banding pattern. Fresh slides of human lymphocytes have been shown to produce good bands for the identification of chromosomes morphology. G-bands by Trypsin using Giemsa (GTG) banding of ag...

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Published inPakistan journal of pharmaceutical sciences Vol. 27; no. 5; p. 1255
Main Authors Sajjad, Naheed, Haque, Sayedul, SBurney, Syed Intesar, Shahid, Syed Muhammad, Zehra, Sitwat, Azhar, Abid
Format Journal Article
LanguageEnglish
Published Pakistan Pakistan Journal of Pharmaceutical Sciences 01.09.2014
Subjects
Azure Stains
Cell culture
Cellular Senescence
Chromosome Banding - methods
Genetic aspects
Humans
Karyotyping
Lymphocytes
Lymphocytes - ultrastructure
Metaphase
Methods
Physiological aspects
Trypsin
Pakistan
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Summary:The identification of chromosomes for routine cytogenetic analysis is based on quality of metaphases and good banding pattern. Fresh slides of human lymphocytes have been shown to produce good bands for the identification of chromosomes morphology. G-bands by Trypsin using Giemsa (GTG) banding of aged slides is generally considered hard to get desired band pattern of chromosomes persistently. The current study is focused on GTG banding of aged slides. A total of 340 subjects including 290 primary infertile and 50 fertile were selected. The blood samples were drawn aseptically for cytogenetic analysis. Lymphocytes were cultured and GTG banding was done on 1440 glass slides. Giemsa trypsin banding of aged slides were done by adjusting average trypsin time for each month according to the slide age and metaphase concentration. Correlation analyses showed a significant and positive correlation between slide ageing and trypsin pre-treatment time. The results of this study suggest that, the fresh and aged human lymphocyte metaphases are equally usable for GTG banding.
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ISSN:1011-601X