Arresting the biosynthesis of Lipid A to hinder Escherichia coli and Pseudomonas aeruginosa through fatty diglyceride

• Published in: Pakistan journal of pharmaceutical sciences Vol. 36; no. 2; pp. 409 - 415
• Main Authors: Khadim Sheikh, Hamdullah, Arshad, Tanzila, Habib, Uzma, Zahra Mirmohammadi, Seyedeh, Usman, Rafia, Mohtasheemul Hassan, Muhammad
• Format: Journal Article
• Language: English
• Published: Pakistan Pakistan Journal of Pharmaceutical Sciences 01.03.2023
• Subjects: Antibacterial agents; Chemical properties; Drug therapy; Endotoxins; Escherichia coli infections; Esters; Health aspects; Microbiological synthesis; Pharmaceutical research; Pseudomonas aeruginosa infections; Pakistan
• ISSN: 1011-601X
• DOI: 10.36721/PJPS.2023.36.2.REG.409-415.1

Lipid A is a fragment of lipopolysaccharide (LPS) in gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa; hence inhibition of its biosynthesis is one of the plausible ways of preventing such bacteria from growth and thus preventing gastrointestinal diseases caused by Escherichia coli and pseudomonas aeruginosa. This research revolves around the development of antibiotic glyceride derivatives for the inhibition of the biosynthesis of lipid A. To target the enzymes involved in the biosynthesis of lipid A, four N,N-dimethylaminobenzoate moiety containing fatty diglyceride derivatives were synthesized through a multi-step synthetic scheme starting from glycerol. The molecular structure of the targeted molecules and synthesized intermediates in the synthetic scheme were confirmed by detailed structural analysis through N-NMR, mass and IR spectroscopic techniques. Antibacterial activity was evaluated against the gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). The derivatives also underwent docking analysis on the pdb's of enzymatic catalysts involved in the biosynthesis of lipid A using AutoDock Vina package. All synthesized fatty esters gave good antibacterial activity and binding energy upto -7 kcal/mol in the docking analysis. A structure-property relationship was established between alkyl chain lengths of diglycerides and their resultant binding energies. These molecules and their resultant activity can assist in further designing and retrosynthesis of molecular derivatives of drug molecules with lipid A biosynthesis as target for its inhibition.