Using Metabolomics to Assist Plant Breeding

Recent methodological advances in both gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) have provided a deep understanding of metabolic regulation occurring in plant cells. The application of these techniques to agricultural systems is, however, subjec...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2264; p. 33
Main Authors Alseekh, Saleh, Fernie, Alisdair R
Format Journal Article
LanguageEnglish
Published United States 2021
Subjects
Chromatography, Liquid - methods
Gas Chromatography-Mass Spectrometry - methods
Metabolome
Metabolomics - methods
Plant Breeding - methods
Plants - metabolism
Quantitative Trait Loci
Metabolomics
Crop improvement
Natural genetic variation
QTL mapping
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Summary:Recent methodological advances in both gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) have provided a deep understanding of metabolic regulation occurring in plant cells. The application of these techniques to agricultural systems is, however, subject to more complex interactions. Here we summarize a step-by-step modern metabolomics methodology that generates metabolome data toward the implementation of metabolomics in crop breeding. We describe a metabolic workflow, and provide guidelines for handling large sample numbers for the specific purpose of metabolic quantitative trait loci approaches.
ISSN:1940-6029
DOI:10.1007/978-1-0716-1201-9_3