Comparison of C18-Carboxypropylbetaine and glass bead DNA extraction methods for detection of Mycobacterium bovis in bovine milk samples and analysis of samples by PCR

• Published in: Applied and environmental microbiology Vol. 64; no. 8; pp. 3099 - 3101
• Main Authors: CORNEJO, B. J, SAHAGUN-RUIZ, A, SUAREZ-GÜEMES, F, THORNTON, C. G, FICHT, T. A, ADAMS, L. G
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.08.1998
• Subjects: Animals; Bacteria; Bacteriological methods and techniques used in bacteriology; Bacteriological Techniques; Bacteriology; Betaine - analogs & derivatives; Biological and medical sciences; Cattle; Deoxyribonucleic acid; Disease; DNA; DNA, Bacterial - isolation & purification; Food Microbiology; Fundamental and applied biological sciences. Psychology; Glass; Microbiology; Microspheres; Milk; Milk - microbiology; Mycobacterium bovis - genetics; Mycobacterium bovis - isolation & purification; Polymerase Chain Reaction - methods; Prospective Studies; Sensitivity and Specificity; Tuberculin Test - veterinary; Tuberculosis, Bovine - diagnosis; Mycobacterium bovis; Method; Polymerase chain reaction; Mycobacteriales; DNA; Pathogenic; Mycobacteriaceae; Bacteria; Cow milk; Actinomycetes; Extraction; Diagnosis; Detection; Comparative study
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.64.8.3099-3101.1998

The purpose of this prospective study was to compare two different milk preparation methods to assay for the presence of Mycobacterium bovis by PCR. Detection by a C18-carboxypropylbetaine (CB-18)-based sample processing method was compared to extraction of DNA from milk with glass beads. Samples from 17 skin test-positive cattle were analyzed. Following CB-18 processing and glass bead extraction, the sensitivity of IS6110-based PCR was 94.1 and 58.8%, respectively (P < 0.025). Because CB-18 processing will permit the proficient use of PCR for diagnosis and surveillance of bovine tuberculosis, it will contribute to the more efficient detection and control of tuberculosis.