Genetic diversity analysis of date palm (Phoenix dactylifera L.) in the Kutch region of India using RAPD and ISSR markers
Date palm is an important fruit crop of the arid and semiarid region. In this paper, two types of DNA based marker RAPD and ISSR were used to characterize 8 date palm genotypes grown in the Kutch region. Amplification of genomic DNA of 8 genotypes using 13 RAPD analyses yielded 88 fragments, of whic...
Saved in:
Published in | Emirates journal of food and agriculture Vol. 25; no. 11; p. 907 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Sofia
Pensoft Publishers
01.11.2013
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Date palm is an important fruit crop of the arid and semiarid region. In this paper, two types of DNA based marker RAPD and ISSR were used to characterize 8 date palm genotypes grown in the Kutch region. Amplification of genomic DNA of 8 genotypes using 13 RAPD analyses yielded 88 fragments, of which 35 were polymorphic, with an average of 2.69 polymorphic fragments per primer. Two ISSR primers produced 13 bands of which 3 were polymorphic. Cluster analysis by UPGMA showed that the dendrogram obtained by RAPD and RAPD + ISSR were similar. Cluster A consisted of Early maturing, Ghanshyam and Late maturing female genotypes with 0.81 to 0.88 Jaccard's similarity range. Cluster B consisted of Seasonal female, Male-1, Male-2, Male-3 and Male-4 genotypes with 0.82 to 0.91 similarity range. Principal coordinate analysis differentiated one group of genotype Male-1 and Male-4 while other genotypes were randomly distributed. |
---|---|
ISSN: | 2079-052X 2079-0538 |
DOI: | 10.9755/ejfa.v25i11.14325 |