Genetic diversity analysis of date palm (Phoenix dactylifera L.) in the Kutch region of India using RAPD and ISSR markers

Date palm is an important fruit crop of the arid and semiarid region. In this paper, two types of DNA based marker RAPD and ISSR were used to characterize 8 date palm genotypes grown in the Kutch region. Amplification of genomic DNA of 8 genotypes using 13 RAPD analyses yielded 88 fragments, of whic...

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Bibliographic Details
Published inEmirates journal of food and agriculture Vol. 25; no. 11; p. 907
Main Authors Srivashtav, V S, Kapadia, C V, Mahatma, M K, Jha, S K, Jha, S, Ahmad, T
Format Journal Article
LanguageEnglish
Published Sofia Pensoft Publishers 01.11.2013
Subjects
Cluster analysis
Crops
Cultivars
Deoxyribonucleic acid
DNA
Females
Genetic diversity
Genomics
Genotype & phenotype
Germplasm
Morphology
Phylogenetics
Polymorphism
Seeds
Studies
India
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Summary:Date palm is an important fruit crop of the arid and semiarid region. In this paper, two types of DNA based marker RAPD and ISSR were used to characterize 8 date palm genotypes grown in the Kutch region. Amplification of genomic DNA of 8 genotypes using 13 RAPD analyses yielded 88 fragments, of which 35 were polymorphic, with an average of 2.69 polymorphic fragments per primer. Two ISSR primers produced 13 bands of which 3 were polymorphic. Cluster analysis by UPGMA showed that the dendrogram obtained by RAPD and RAPD + ISSR were similar. Cluster A consisted of Early maturing, Ghanshyam and Late maturing female genotypes with 0.81 to 0.88 Jaccard's similarity range. Cluster B consisted of Seasonal female, Male-1, Male-2, Male-3 and Male-4 genotypes with 0.82 to 0.91 similarity range. Principal coordinate analysis differentiated one group of genotype Male-1 and Male-4 while other genotypes were randomly distributed.
ISSN:2079-052X
2079-0538
DOI:10.9755/ejfa.v25i11.14325