QTLs Associated with Agronomic Traits in the Cutler x AC Barrie Spring Wheat Mapping Population Using Single Nucleotide Polymorphic Markers

• Published in: PloS one Vol. 11; no. 8; p. e0160623
• Main Authors: Perez-Lara, Enid, Semagn, Kassa, Chen, Hua, Iqbal, Muhammad, N'Diaye, Amidou, Kamran, Atif, Navabi, Alireza, Pozniak, Curtis, Spaner, Dean
• Format: Journal Article
• Language: English
• Published: Public Library of Science 11.08.2016
• Subjects: Genetic aspects; Growth; Physiological aspects; Quantitative trait loci; Single nucleotide polymorphisms; Wheat; Canada
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0160623

We recently reported three earliness per se quantitative trait loci (QTL) associated with flowering and maturity in a recombinant inbred lines (RILs) population derived from a cross between the spring wheat (Triticum aestivum L.) cultivars 'Cutler' and 'AC Barrie' using 488 microsatellite and diversity arrays technology (DArT) markers. Here, we present QTLs associated with flowering time, maturity, plant height, and grain yield using high density single nucleotide polymorphic (SNP) markers in the same population. A mapping population of 158 RILs and the two parents were evaluated at five environments for flowering, maturity, plant height and grain yield under field conditions, at two greenhouse environments for flowering, and genotyped with a subset of 1809 SNPs out of the 90K SNP array and 2 functional markers (Ppd-D1 and Rht-D1). Using composite interval mapping on the combined phenotype data across all environments, we identified a total of 19 QTLs associated with flowering time in greenhouse (5), and field (6) conditions, maturity (5), grain yield (2) and plant height (1). We mapped these QTLs on 8 chromosomes and they individually explained between 6.3 and 37.8% of the phenotypic variation. Four of the 19 QTLs were associated with multiple traits, including a QTL on 2D associated with flowering, maturity and grain yield; two QTLs on 4A and 7A associated with flowering and maturity, and another QTL on 4D associated with maturity and plant height. However, only the QTLs on both 2D and 4D had major effects, and they mapped adjacent to well-known photoperiod response Ppd-D1 and height reducing Rht-D1 genes, respectively. The QTL on 2D reduced flowering and maturity time up to 5 days with a yield penalty of 436 kg ha.sup.-1, while the QTL on 4D reduced plant height by 13 cm, but increased maturity by 2 days. The high density SNPs allowed us to map eight moderate effect, two major effect, and nine minor effect QTLs that were not identified in our previous study using microsatellite and DArT markers. Results from this study provide additional information to wheat researchers developing early maturing and short stature spring wheat cultivars.