Sensitive fluorescent imaging of Salmonella enteritidis and Salmonella typhimurium using a polyvalent directed peptide polymer

• Published in: Mikrochimica acta (1966) Vol. 184; no. 8; pp. 2611 - 2620
• Main Authors: Lee, Sang-Choon, Kim, Min-Seo, Yoo, Ki-Chun, Ha, Na-Reum, Moon, Ji-Young, Lee, Su-Jae, Yoon, Moon-Young
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.08.2017; Springer; Springer Nature B.V
• Subjects: Affinity; Analytical Chemistry; Binding sites; Characterization and Evaluation of Materials; Chemical synthesis; Chemistry; Chemistry and Materials Science; Comparative analysis; Diagnostic systems; Eggs; Fluorescein; Fluorescence; Fluorescence microscopy; Fluoroscopic imaging; Imaging; Lysine; Microengineering; Nanochemistry; Nanotechnology; Original Paper; Peptides; Phages; Polymer industry; Polymers; Poultry; Salmonella; Salmonella enteritidis; Salmonella food poisoning; Salmonella typhimurium; Fluorescein isothiocyanate; Cell-phage display; Succinimidyl 3-(2-pyridyldithio)propionate; Polyvalant directed peptide polymer; Food poisoning; RT-PCR
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-017-2240-1

The authors describe three fluorescein-conjugated peptides generated by cell-phage display for use as a diagnostic probes for fluorescent detection and imaging of Salmonella enteritidis and Salmonella typhimurium . The authors also designed a polyvalent-directed peptide polymer synthesized with poly-D-lysine and bifunctional succinimidyl 3-(2-pyridyldithio)propionate with an affinity and sensitivity that is higher by more than an order of magnitude compared to single peptides due to multiple binding site interactions. In order to establish a diagnostic system for food poisoning, imaging analysis was performed using fluorescence microscopy. The limit of detection of the diagnostic system based on polyvalent directed peptide interaction is 10 2 colony-forming units per mL for Salmonella . Graphical abstract Schematic of a fluorescent method for detection and imaging of Salmonella enteritidis and Salmonella typhimurium by using a fluorescein labeled polyvalent-directed peptide polymer (PDPP) with a high affinity and sensitivity as a diagnostic probe. The system uses a microplate reader and was applied to the detection of food poisoning.