Enhancement of sporulation and crystal toxin production by cornsteep liquor feeding during intermittent fed-batch culture of Bacillus sphaericus 2362

A medium containing cornsteep liquor (CSL) as a replacement for yeast extract (YE) enhanced the sporulation of Bacillus sphaericus 2362. Fed-batch culture with combinations of YE and CSL together with acetate as carbon source resulted in a cell mass of 13.0 g/L and a high spore count of 16.4 X 10 su...

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Bibliographic Details
Published inBiotechnology letters Vol. 20; no. 2; pp. 165 - 168
Main Authors SASAKI, K, JIAVIRIYABOONYA, S, ROGERS, P. L
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.02.1998
Subjects
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Culture medium
Toxin
Bacillales
Sporulation
Production
Microorganism culture
Bacteria
Bacillaceae
Bacillus sphaericus
Semicontinuous
Optimization
Spores
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Summary:A medium containing cornsteep liquor (CSL) as a replacement for yeast extract (YE) enhanced the sporulation of Bacillus sphaericus 2362. Fed-batch culture with combinations of YE and CSL together with acetate as carbon source resulted in a cell mass of 13.0 g/L and a high spore count of 16.4 X 10 super(9)/mL. This compared to an acetate, YE based medium which produced a similar cell concentration but only 4 X 10 super(9) spores/mL. Western blot analysis indicated significant toxin production in the former case.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005332609136