Molecular and Phenotypic Characterization of Two Bacteria, Photorhabdus luminescens subsp. akhurstii HRM1 and HS1 Isolated from Two Entomopathogenic Nematodes, Heterorhabditis indica RM1 and Heterorhabditis sp. S1

The present study was aimed at molecular and phenotypic characterization of two new bacterial strains isolated from entomopathogenic nematodes, Heterorhabditis indica RM1 from Alexandria, northern Egypt and Heterorhabditis sp. S1 from RasSidr, South Sinai, eastern Egypt. These entomopathogenic bacte...

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Published inPakistan journal of zoology Vol. 48; no. 1
Main Authors El-Sadawy, Hanan A, Forst, Steven, Abouelhag, Hussien A, Ahmed, Ashraf M, Alajmi, Reem A, Ayaad, Tahany H
Format Journal Article
LanguageEnglish
Published Lahore Knowledge Bylanes 29.02.2016
AsiaNet Pakistan (Pvt) Ltd
Subjects
Antibiotics
Bacteria
Deoxyribonucleic acid
DNA
Entomopathogenic nematodes
Gene sequencing
Heterorhabditidae
Heterorhabditis indica
Identification and classification
Insects
Maximum likelihood method
Microorganisms
Nematoda
Nematodes
Pathogenic bacteria
Photorhabdus luminescens akhurstii
Phylogenetics
Phylogeny
Rhabditids
rRNA 16S
Strains (organisms)
Taxonomy
Toxins
Xenorhabdus
Egypt
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Summary:The present study was aimed at molecular and phenotypic characterization of two new bacterial strains isolated from entomopathogenic nematodes, Heterorhabditis indica RM1 from Alexandria, northern Egypt and Heterorhabditis sp. S1 from RasSidr, South Sinai, eastern Egypt. These entomopathogenic bacteria secrete lethal toxins that rapidly kill the insect host within 48h. They also produce various antibiotics that suppress infection by other microorganisms. Three complementary approaches including16S rRNA gene sequencing, phenotypic and phylogenetic analysis by maximum likelihood method and chemical characterization, were used to identify these new isolates. Current investigations revealed that these two isolates had noteworthy differences, particularly in their antibiotic production spectrum from P. luminescens subsp. akhurstii 1S5; however, they maintain the major characters that define them from other species. A minor difference from regular P. luminescens subsp. akhurstii was obtained by 16S rRNA gene sequencing, which resulted in two new type strains: Photorhabdus luminescens subsp. akhurstii HRM1KC237382 and Photorhabdus luminescens subsp. akhurstii HS1KC237383.
ISSN:0030-9923