The Use of High Performance Liquid Chromatography for the Characterization of the Unfolding and Aggregation of Dairy Proteins

High-performance liquid chromatography (HPLC) is routinely used to identify and characterize proteins. HPLC can help to understand protein aggregation processes in dairy products, which are induced by common industrial processing steps such as heat treatment. In this chapter, three complementary chr...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2039; p. 103
Main Authors Gaspard, Sophie Jeanne, Brodkorb, André
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Chromatography, High Pressure Liquid - methods
Chromatography, Reverse-Phase - methods
Protein Aggregates - physiology
Protein Folding
Proteins - chemistry
Aggregation
Proteins
Size exclusion chromatography
Reversed-phase chromatography
Denaturation
HPLC
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Summary:High-performance liquid chromatography (HPLC) is routinely used to identify and characterize proteins. HPLC can help to understand protein aggregation processes in dairy products, which are induced by common industrial processing steps such as heat treatment. In this chapter, three complementary chromatographic methods are described, which are based on the principles of size exclusion and reversed-phase chromatography. These methods are used to determine the degree of denaturation and aggregation of proteins, and estimate the molecular weight of these aggregates.
ISSN:1940-6029
DOI:10.1007/978-1-4939-9678-0_8