The continuous hydrolysis of geniposide to genipin using immobilized β-glucosidase or calcium alginate gel

• Published in: Biotechnology letters Vol. 9; no. 10; pp. 697 - 702
• Main Authors: FUJIKAWA, S, YOKOTA, T, KOGA, K, KUMADA, J.-I
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.10.1987
• Subjects: beta -D-glucosidase; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Gardenia jasminoides; genipin; geniposide; immobilized enzymes; Methods. Procedures. Technologies; Intermediate product; β-D-Glucosidase; Continuous; Enzymatic catalysis; Alginates; Hydrolysis; Synthesis; Operating stability; Biotransformation; Production; Pigments; Kinetics; Immobilized enzyme

The reaction velocity of immobilized beta -glucosidase was approximated by the first-order reaction kinetics. A plug flow reactor was used for continuous hydrolysis of geniposide with this immobilized enzyme. The activity of this immobilized enzyme was retained 100% for 600 h. The amount of genipin...