The continuous hydrolysis of geniposide to genipin using immobilized β-glucosidase or calcium alginate gel

The reaction velocity of immobilized beta -glucosidase was approximated by the first-order reaction kinetics. A plug flow reactor was used for continuous hydrolysis of geniposide with this immobilized enzyme. The activity of this immobilized enzyme was retained 100% for 600 h. The amount of genipin...

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Bibliographic Details
Published inBiotechnology letters Vol. 9; no. 10; pp. 697 - 702
Main Authors FUJIKAWA, S, YOKOTA, T, KOGA, K, KUMADA, J.-I
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.10.1987
Subjects
beta -D-glucosidase
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Gardenia jasminoides
genipin
geniposide
immobilized enzymes
Methods. Procedures. Technologies
Intermediate product
β-D-Glucosidase
Continuous
Enzymatic catalysis
Alginates
Hydrolysis
Synthesis
Operating stability
Biotransformation
Production
Pigments
Kinetics
Immobilized enzyme
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Summary:The reaction velocity of immobilized beta -glucosidase was approximated by the first-order reaction kinetics. A plug flow reactor was used for continuous hydrolysis of geniposide with this immobilized enzyme. The activity of this immobilized enzyme was retained 100% for 600 h. The amount of genipin formed by using the immobilized enzyme was 17 fold that formed using the native enzyme without reuse. Using immobilized enzyme, purity and yield of genipin, which is a hydrolyzate of geniposide, was improved comparing with the native enzyme.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0141-5492
DOI:10.1007/BF01024600