The continuous hydrolysis of geniposide to genipin using immobilized β-glucosidase or calcium alginate gel
The reaction velocity of immobilized beta -glucosidase was approximated by the first-order reaction kinetics. A plug flow reactor was used for continuous hydrolysis of geniposide with this immobilized enzyme. The activity of this immobilized enzyme was retained 100% for 600 h. The amount of genipin...
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| Published in | Biotechnology letters Vol. 9; no. 10; pp. 697 - 702 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Dordrecht
Springer
01.10.1987
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| Subjects | |
| Online Access | Get full text |
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| Abstract | The reaction velocity of immobilized beta -glucosidase was approximated by the first-order reaction kinetics. A plug flow reactor was used for continuous hydrolysis of geniposide with this immobilized enzyme. The activity of this immobilized enzyme was retained 100% for 600 h. The amount of genipin formed by using the immobilized enzyme was 17 fold that formed using the native enzyme without reuse. Using immobilized enzyme, purity and yield of genipin, which is a hydrolyzate of geniposide, was improved comparing with the native enzyme. |
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| AbstractList | The reaction velocity of immobilized beta -glucosidase was approximated by the first-order reaction kinetics. A plug flow reactor was used for continuous hydrolysis of geniposide with this immobilized enzyme. The activity of this immobilized enzyme was retained 100% for 600 h. The amount of genipin formed by using the immobilized enzyme was 17 fold that formed using the native enzyme without reuse. Using immobilized enzyme, purity and yield of genipin, which is a hydrolyzate of geniposide, was improved comparing with the native enzyme. |
| Author | KOGA, K FUJIKAWA, S YOKOTA, T KUMADA, J.-I |
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| Keywords | Intermediate product β-D-Glucosidase Continuous Enzymatic catalysis Alginates Hydrolysis Synthesis Operating stability Biotransformation Production Pigments Kinetics Immobilized enzyme |
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| SubjectTerms | beta -D-glucosidase Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Gardenia jasminoides genipin geniposide immobilized enzymes Methods. Procedures. Technologies |
| Title | The continuous hydrolysis of geniposide to genipin using immobilized β-glucosidase or calcium alginate gel |
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