Immobilization of hog pancreas lipase in macroporous poly(vinyl alcohol)-cryogel carrier for the biocatalysis in water-poor media

Hog pancreas lipase was covalently attached to the beads of poly(vinyl alcohol)-cryogel - a macroporous hydrogel prepared by means of freeze-thaw technique. The immobilized biocatalyst thus obtained was examined in the reaction of enantioselective hydrolysis of the ethyl ester of N-benzylidene deriv...

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Published inBiotechnology letters Vol. 22; no. 7; pp. 551 - 554
Main Authors Plieva, Fm, Kochetkov, Ka, Singh, Ishwar, Parmar, Vs, Belokon', Yun, Lozinsky, Vi
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.04.2000
Subjects
Amino acids
Hogs
polyvinyl alcohol
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Summary:Hog pancreas lipase was covalently attached to the beads of poly(vinyl alcohol)-cryogel - a macroporous hydrogel prepared by means of freeze-thaw technique. The immobilized biocatalyst thus obtained was examined in the reaction of enantioselective hydrolysis of the ethyl ester of N-benzylidene derivative of DL-phehylalanine in the medium of acetonitrile (contained 5 vol.% of water without any buffers). Eighty-three %-enantiomeric excess of the l-amino acid was reached after 144 h. Virtually the same result was obtained in the repeated use of the same immobilized biocatalyst after its 6-months-storing in a refrigerator.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005660125927