Design and fabrication of electrospun SBA-15-incorporated PVA with curcumin: a biomimetic nanoscaffold for skin tissue engineering

• Published in: Biomedical materials (Bristol) Vol. 15; no. 3; p. 035009
• Main Authors: Rathinavel, Saranya, Ekambaram, Shoba, Korrapati, Purna Sai, Sangeetha, Dharmalingam
• Format: Journal Article
• Language: English
• Published: England 05.03.2020
• Subjects: Adsorption; Animals; Anti-Bacterial Agents - chemistry; Anti-Infective Agents - chemistry; Anti-Inflammatory Agents - chemistry; Biocompatible Materials - chemistry; Biomimetics; Cell Adhesion; Cell Line; Cell Line, Tumor; Curcumin - chemistry; Drug Delivery Systems; Female; Humans; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Nanofibers - chemistry; Nitrogen - chemistry; Polyvinyl Alcohol - chemistry; Rats; Rats, Wistar; Silicon Dioxide - chemistry; Skin - drug effects; Spectroscopy, Fourier Transform Infrared; Tissue Engineering - methods; Tissue Scaffolds; Wound Healing; X-Ray Diffraction
• ISSN: 1748-605X
• DOI: 10.1088/1748-605X/ab6b2f

Fabricating and designing a scaffold is a complex and highly challenging process in the current scenario. The present study deals with the design and fabrication of electrospun Santa Barbara Amorphous (SBA)-15-incorporated polyvinyl alcohol (PVA) with curcumin, which can be used as a biomimetic nanoscaffold for skin tissue engineering. Curcumin was selected due to its effective anti-microbial and anti-inflammatory properties. SBA-15 was selected for its characteristic drug-carrying potential. Fourier transform infrared spectroscopy and x-ray diffraction characterizations of the fabricated nanofiber demonstrated the interaction of PVA, SBA-15 and curcumin. The scanning electron microscopy results depicted that the nanofiber was highly interconnected with a porous structure mimicking the extracellular matrix. The nanofibrous scaffold showed a higher percentage of cell migration, proliferation, cytocompatibility and biocompatibility with absence of cytotoxicity which was evidenced from the results of MTT assay, cell adhesion and live/dead assay using HaCaT cells. The results of the anti-bacterial test depicted that the synthesized nanofiber forms a potent material for skin wound-healing therapeutics. The in vitro drug release study performed over a period of 80 h revealed a sustained release pattern of curcumin from the SBA-15-incorporated PVA nanofiber. Finally, the in vivo results confirmed that SBA-15-incorporated PVA nanofiber with curcumin showed efficient wound-healing activities.