High resolution glycopeptide mapping of EPO using an Agilent AdvanceBio Peptide Mapping column

Introduction Peptide fragment analysis by liquid chromatography-mass spectrometry (LC-MS) is a crucial requirement for the release of biotherapeutic products and plays an important role in completing the protein characterization strategy. Extended dynamic range mode (2 GHz) Chromatographic Condition...

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Bibliographic Details
Published inLC GC North America Vol. 31; no. 6; p. S27
Main Authors Martosella, James, Duong, Phu, Zhu, Alex
Format Magazine Article Trade Publication Article
LanguageEnglish
Published Monmouth Junction MJH Life Sciences Media 01.06.2013
MultiMedia Healthcare Inc
Subjects
Acids
Chromatography
Glycoproteins
Mapping
Mass spectrometry
Peptides
Proteins
Retention
Scientific imaging
United States
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Summary:Introduction Peptide fragment analysis by liquid chromatography-mass spectrometry (LC-MS) is a crucial requirement for the release of biotherapeutic products and plays an important role in completing the protein characterization strategy. Extended dynamic range mode (2 GHz) Chromatographic Conditions Column: Agilent AdvanceBio Peptide Mapping, 2.1 x 250 mm, 2.7 pm (p/n 651750-902) Eluent: A, H20 + 0.1% formic acid (v/v); B, acetonitrile + 0.1% formic acid (v/v) Injection volume: 5 pL (2 pg/pL) Flow rate: 0.4 mL/min Temperature: 55 °C Results and Discussion Optimized rhEPO High Pressure Liquid Chromatography (HPLC) Peptide Mapping with an AdvanceBio Peptide Mapping Column Systematic method development was performed for mapping the rhEPO digest to determine the best resolved separation under water/acetonitrile (ACN) conditions.
ISSN:1527-5949
1939-1889