Single-Spore Extraction for Genetic Analyses of Arbuscular Mycorrhizal Fungi

Biomass of arbuscular mycorrhizal fungi (AMF, Glomeromycota) is often only available in small quantities as these fungi are obligate biotrophs and many species are difficult to cultivate under controlled conditions. Here, I describe a simple, efficient approach to produce crude extracts from single...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2146; p. 93
Main Author Redecker, Dirk
Format Journal Article
LanguageEnglish
Published United States 2020
Subjects
Glomeromycota - growth & development
Glomeromycota - isolation & purification
Mycorrhizae - growth & development
Plant Roots - microbiology
Single-Cell Analysis - methods
Soil Microbiology
Spores, Fungal - growth & development
Spores, Fungal - isolation & purification
Symbiosis - genetics
Genotyping
Glomeromycota
Single-spore extraction
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Summary:Biomass of arbuscular mycorrhizal fungi (AMF, Glomeromycota) is often only available in small quantities as these fungi are obligate biotrophs and many species are difficult to cultivate under controlled conditions. Here, I describe a simple, efficient approach to produce crude extracts from single or a small number of spores that can be used for genotyping AMF.
ISSN:1940-6029
DOI:10.1007/978-1-0716-0603-2_8