Gene expression RNA interference in adult mice

RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcr...

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Bibliographic Details
Published inNature (London) Vol. 418; no. 6893; pp. 38 - 39
Main Authors Kay, Mark A, McCaffrey, Anton P, Meuse, Leonard, Pham, Thu-Thao T, Conklin, Douglas S, Hannon, Gregory J
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group 04.07.2002
Subjects
Deoxyribonucleic acid
DNA
Gene expression
Hepatitis
Hepatitis C
Medical research
Vertebrates
United States > US
Utah
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Summary:RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo.
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ISSN:0028-0836
1476-4687
DOI:10.1038/418038a